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gpr [2019-08-07 13:20:25]
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gpr [2019-08-07 13:20:25]

germination protease (degradation of SASPs)
Locus
BSU_25540
Isoelectric point
5.20
Molecular weight
40.12 kDa
Protein length
368 aa Sequence Blast
Gene length
1107 bp Sequence Blast
Function
degradation of SASPs
Product
germination protease (degradation of SASPs)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Proteolysis during sporulation/ germination
  • Lifestyles → Sporulation → Germination → Additional germination proteins

    • Gene

    Coordinates
    2,634,505 2,635,611

    The protein

    Catalyzed reaction/ biological activity

  • degradation of small, acid-soluble spore proteins (SASPs) PubMed

    • Protein family

  • peptidase A25 family (single member, according to UniProt)

    • Structure

  • 1C8B (from B. megaterium, 68% identity) PubMed

    • Localization

  • inner spore membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    gpr-spoIIP
    Description
    PubMed

    Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • SigG: sigma factor, PubMed, in SigG regulon

    • Regulatory mechanism

  • SpoVT: repression, PubMed, in SpoVT regulon

    • Regulation

  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed
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    Biological materials

    Mutant

  • BKE25540 (gpr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
  • BKK25540 (gpr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT

    • References

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